ESR9 will investigate applications for the synthesis of hydroxyketones, as they are complex models carrying multiple chiral elements. The researcher will evaluate wild type and variants of transketolases and glyoxylate carboligases (GLCs) that are industrially applicable. They will use directed evolution to expand the substrate scopes of these enzymes towards donor and acceptor substrates and generate libraries that can be screened using high-throughput assays, in order to retrieve the best variants for biocatalytic processes.

Project information

Workpackage: WP2

Project title: TDP dependent transketolases and glyoxylate carboligases for synthesis of chiral polyols

Host institution: Université Clermont-Auvergne

Secondments: TU Darmstadt, Georg-August-Universität Göttingen and BASF SE

Supervisor: Laurence Hecquet