ESR7 will develop transketolase variants with activity against non-natural electrophiles using directed evolution. The goal with is to broaden transketolase catalysis for novel carboligations. The researchers enrolled will characterise these variants kinetically for their substrate scope and will use them for preparative-scale synthesis. Jointly with ESR6 and ESR8 the most interesting enzymes developed will be subjected to X-ray structure determination.

Project information

Workpackage: WP2

Project title: Engineering Transketolase for novel reactivities

Host institution: TU Darmstadt

Secondments: Albert-Ludwigs-Universität Freiburg, Georg-August-Universität Göttingen and AstraZeneca

Supervisor: Wolf-Dieter Fessner