ESR5 is set to use sequence-based metagenome mining to identify “split” transketolases from mesophile and thermophile genomes. Once this task is completed, the researcher will clone and/or express several of these enzymes and characterise them for substrate specificity, substrate tolerance and stability. Finally, the project includes the determination of the structure of the best performing enzymes through X-ray crystallography.

Project information

Workpackage: WP2

Project title: Metagenomics to discover new 'split' transketolases

Host institution: University College London

Secondments: TU Darmstadt, Université Clermont-Auvergne and Johnson Matthey

Supervisor: John Ward