ESR3 will focus on the development of standard kinetic assays for enzyme hit characterisation based on absorption. The researcher that enrols in this project will address the elucidation of the application scope of novel PEP dependent synthases. Specifically, they will identify sequences of PEP lyases in public databases, express and screen new lyases for substrate specificity, develop and validate new assays for lyases activity and verify enzyme stereoselectivity by preparative synthesis. Finally, they will determine the structure of the most interesting enzyme detected.

Project information

Workpackage: WP3

Project title: Discovery of new PEP lyases

Host institution: TU Darmstadt

Secondments: Georg-August-Universität Göttingen and Prozomix Ltd

Supervisor: Wolf-Dieter Fessner