This project will address development opportunities of Ultra-High Throughput Screening assays for enzymatic carboligation. The researcher ESR2 will establish microfluidic workflows to screen the natural and artificial diversity of carboligases. This will include a systematic survey of fluorophores, additives, oils and surfactants to reduce leakage, the adaptation of existing carboligases assays to microfluidics and an initial characterisation of the enzymes.

Project information

Workpackage: WP2

Project title: Microfluidic high-throughput screening of C-C bond forming enzymes

Host institution: Universidad Autónoma de Madrid

Secondments: Prozomix Ltd and TU Darmstadt

Supervisor: Aurelio Hidalgo