This project investigates the promiscuous activity of 4-OT and related enzymes to promote the asymmetric addition of nitroalkanes to a wide variety of α,β-unsaturated aldehydes. For this purpose, the researcher enrolled will optimise the enzymes for reactions that yield interesting GABA precursors by using different tools of protein engineering. They will afterwards characterise the artificial Michaelases in terms of structure-function relationships, using techniques such as DNA sequencing, protein purification or X-ray structure analysis.

Project information

Workpackage: WP3

Project title: Engineering of enantioselective Michaelases

Host institution: Rijksuniversiteit Groningen

Secondments: University of Zagreb and Enzymicals AG

Supervisor: Gerrit J. Poelarends