ESR13 investigates 4-oxalocrotonate tautomerase (4-OT) promoted aldol addition of acetaldehyde to a variety of benzaldehydes looking for efficiency, stereochemistry and product selectivity. The researcher will generate mutability landscapes for this enzyme to develop tailor-made aldolases with enhanced activity and selectivity and will harness the best performing enzymes in (chemo) enzymatic cascades to prepare todiols and aminoalcohols. In addition, redesigned natural aldolases will also be tested as potential catalysts for these and related reactions. Moreover, the researcher will oversee characterisation studies of these enzymes.

Project information

Workpackage: WP3

Project title: Exploiting enzyme promiscuity to develop novel aldolases

Host institution: Rijksuniversiteit Groningen

Secondments: Prozomix Ltd and TU Darmstadt

Supervisor: Gerrit J. Poelarends