This project will focus on the rational redesign of a fructose-6-phosphate aldolase for promiscuous substrate specificities. The research will start from an exhaustive characterization of the parent catalyst in terms of structure and kinetic profile. This will render the protein hotspots that are critical for substrate binding and specificity that will subsequently serve as a base for engineering novel biocatalytic applications.

Project information

Workpackage: WP3

Project title: Structure-guided design of novel thermophilic aldolases

Host institution: Georg-August-Universität Göttingen

Secondments: TU Darmstadt and BASF SE

Supervisor: Kai Tittmann