ESR10 focuses on mapping the spectrum of substrate acceptance of different type of TDP dependent enzymes from an industrial perspective. The goal of this task is to obtain a toolbox of catalysts that cover attractive reactions. The researcher in charge of this project will recruit different classes of carboligases, both novel and previously identified in the literature. This will include cloning and expressing the enzymes, screening them to determine their substrate scope, engineering the protein to address limitations and investigate how to intensify the reaction and scaling it up to an industrial level.

Project information

Workpackage: WP2

Project title: Screening of C-C bond forming enzymes and reaction intensification for synthesis of chiral intermediates

Host institution: Johnson Matthey

Secondments: University College London and Georg-August-Universität Göttingen

Supervisor: Serena Bisagni